Kevin-Prince Boateng made his Barcelona debut as his side fell to a 2-0 defeat against Sevilla in the quarter-final of the Copa del Rey first leg on Wednesday night.
With Messi rested, Boateng was given the nod by coach Ernesto Valverde but he was replaced by Luis Suarez in the second half.
Pablo Sarabia blasted home Quincy Promes' cross to put the hosts ahead.
And Wissam Ben Yedder slid in at the back post following Ever Banega's deflected shot to double their lead.
The Catalans have won the past four Spanish Cups, beating Sevilla in two of those finals - 2-0 in 2016 and 5-0 in 2018. The last time they failed to reach the final was 2012-13.
Real Madrid host Girona in their quarter-final first leg on Thursday