A No 10 source has said a Brexit deal is "essentially impossible" after a call between the PM and Angela Merkel.
Boris Johnson and the German chancellor spoke earlier about the proposals he had put forward to the EU - but the source said she made clear a deal based on them was "overwhelmingly unlikely".
Mrs Merkel's office said it would not comment on "private" conversations.
But the BBC's Adam Fleming said there was "scepticism" within the EU that Mrs Merkel would have used such language.
And the EU's top official warned the UK against a "stupid blame game".
President of the European Council Donald Tusk sent a public tweet to Mr Johnson, telling him "the future of Europe and the UK" was at stake.
With efforts to get a deal by the end of the month on an apparent knife edge, Mr Johnson and his Irish counterpart Leo Varadkar have said they hope to meet later in the week.
But Mr Varadkar told an interviewer on Tuesday evening he thought it would be "very difficult" to secure an agreement by next week.
He said the UK had "repudiated" the deal negotiated previously with Theresa May's government and had "sort of put half of that now back on the table, and are saying that's a concession. And of course it isn't, really".
And following talks in Downing Street, the president of the European Parliament said there had been "no progress" and MEPs would not agree to a compromise deal "at any price".
David Sassoli said the UK's new proposed customs arrangements for Northern Ireland were a "long way from something to which the Parliament could agree".
The president of the European Parliament said the EU faced a no-deal exit or a further delay
Amid frantic diplomatic manoeuvring in European capitals, details of a call earlier on Tuesday between the UK and German leaders have reignited tensions across the continent.
The No 10 source suggested Mrs Merkel told her counterpart the only way to break the deadlock was for Northern Ireland to stay in the customs union and for it to permanently accept EU single market rules on trade in goods.
This, the source said, marked a shift in Germany's approach and made a negotiated deal "essentially impossible".
The prime minister's official spokesman said the conversation had been "frank" but denied the negotiations were all but over.
Norbert Rottgen, an ally of the chancellor who is chair of the Bundestag's Foreign Affairs Committee, said there was "no new German position".
He tweeted that a deal based on the UK's latest proposals had "been unrealistic from the beginning and yet the EU has been willing to engage".
The BBC's Europe editor Katya Adler said it was "no secret" Berlin found the UK's proposed new customs solution for Northern Ireland problematic.
While Berlin had not given up hope, she said the chances of a no-deal exit were rising again as the nature of the UK's proposals made any compromise very difficult.
Under Mr Johnson's proposals, which he calls a "broad landing zone" for a new deal with the EU:

- Northern Ireland would leave the EU's customs union alongside the rest of the UK, at the start of 2021
- But Northern Ireland would continue to apply EU legislation relating to agricultural and other products, if the Northern Ireland Assembly approves
- This arrangement could, in theory, continue indefinitely, but the consent of Northern Ireland's politicians would have to be sought every four years
- Customs checks on goods traded between the UK and EU would be "decentralised", with paperwork submitted electronically and only a "very small number" of physical checks
- These checks should take place away from the border itself, at business premises or at "other points in the supply chain"
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