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Fabrice Muamba's heart condition is "showing small signs of improvement" following his cardiac arrest.
A Bolton statement said: "His heart is now beating without the help of medication and he is also moving his arms and legs.
"However, his long-term prognosis will remain unclear for some time.
"He is still critically ill and will continue to be closely monitored and treated by staff in The London Chest Hospital's intensive care unit."
The midfielder, 23, has been at the hospital's heart attack centre after suffering the cardiac arrest during Saturday's FA Cup tie at Tottenham.
Bolton chairman Phil Gartside and manager Owen Coyle visited the player on Monday morning.
In a statement on the club's website, Gartside said: "The staff at the London Chest Hospital have been nothing short of exceptional and I would like to thank them all at this ongoing critical time.
"The support we had from Tottenham and our own fans was fantastic - the staff, the fans, the players and the officials at the game - it was unbelievable."
Muamba's fiancee Shauna Magunda, the mother of his son Joshua, has thanked well-wishers for their support.
"Where there is life there is hope," she tweeted on Monday.
Bolton manager Owen Coyle said: "From our end, and on behalf of the family, we certainly want to say thanks to everybody.
"Everybody is praying for Fabrice which is very important and that has been a real source of strength to the family."
Bolton's Premier League game at Aston Villa on Tuesday has been postponed.
Medics spent six minutes trying to resuscitate the former England Under-21 international on the pitch after he fell to the ground with no other players around him.
The score was 1-1 when the quarter-final was abandoned after 41 minutes.
The Press Association reports that it took medical staff two hours to get Muamba breathing again.
Tottenham chairman Daniel Levy said in a statement: "Our thoughts are with Fabrice's family and Bolton Wanderers, and we are all willing him to pull through. Events such as this put everything into perspective."
"Too often we read the negatives about football and yet, at a time of intense emergency and uncertainty, we saw the true humanity and empathy of the footballing family."
Spurs manager Harry Redknapp added: "Football is the last thing on anyone's mind when an awful situation such as this happens. All we are thinking about now is Fabrice and his family."
Medical staff gave mouth-to-mouth resuscitation to Muamba, who was born in Zaire, now Democratic Republic of Congo, but moved to England aged 11, and tried to revive him with a defibrillator.
In total, six medics were treating the player, who joined Wanderers from Birmingham in 2008 and has made 148 appearances for the club.
Both sets of supporters chanted Muamba's name as he was taken off the pitch on a stretcher.
Referee Howard Webb called the visibly shaken players off the field after consulting with Coyle and Redknapp. After Muamba had been taken to hospital, the match was abandoned.
The former Arsenal midfielder was accompanied in the ambulance by Coyle and club captain Kevin Davies.
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