Hearts of Oak joined the list of second round casualties in the MTN FA Cup as they became the second Premier League team to have been eliminated.

And they join a list of five elite casualties so far, coming a day after Wa All Stars were eliminated from the competition.

Other Premier League teams who have been dumped out of the competition by teams from the lower divisions are Ebusua Dwarfs, Heart of Lions and Amidaus Professionals.

The Phobians were kicked out on Sunday after a 2-1 defeat to Division One league side, Gold Stars in Bibiani.

It will come as a blow to their title ambitions as their elimination leaves Mohammed Polo with only one silverware - Premier League title - to compete for now.

The defeat also represents their second-straight loss in a competitive match following their 3-0 loss to Wa All Stars seven days ago in the Premier League.

However, unlike the Phobians, reigning Cup holders Medeama, AshantiGold and Berekum Chelsea survived testing battles to progress to the third round.

Medeama defeated Division Two side, Score FC 2-1 in Koforidua while AshantiGold were 3-1 winners against former premiership side, B.A. United.

At the Golden City, Berekum Chelsea won 3-0 over Wenchi United.

Round of 64 Results

 Berekum Chelsea 3-0 Wenchi United

Gold Stars 2-1 Accra Hearts of Oak

AshantiGold 3-1 BA United

Score FC 1-2 Medeama

Dolphins FC 2-2 (4-2 pen) Guan United

Prestige FC 0-1 Istanbul FC

New Edubiase 4-0 Bolga Man City

Tarkwa United 2-0 Hearts of Lions

Ebusua Dwarfs 1-1(4-5 pen) First Klass FC

Okwawu United 1-0 Real Athletico

King Faisal 3-1 Tuobodom Santos

Starke FC 1-2 Hasaacas

Pure Joy 2-1 Ajax Fc

Bechem United 4-0 Cape Coast Strikers

Amidaus Professionals 0-2 Danbort FC